Tested Applications
| Positive WB detected in | PMA treated THP-1 cells, THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83438-6-RR targets TREM2 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg0747 Product name: Recombinant human TREM2 protein Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 19-174 aa of BC032362 Sequence: HNTTVFQGVAGQSLQVSCPYDSMKHWGRRKAWCRQLGEKGPCQRVVSTHNLWLLSFLRRWNGSTAITDDTLGGTLTITLRNLQPHDAGLYQCQSLHGSEADTLRKVLVEVLADPLDHRDAGDLWFPGESESFEDAHVEHSISRPSQGSHLPSCLSK Predict reactive species |
| Full Name | triggering receptor expressed on myeloid cells 2 |
| Calculated Molecular Weight | 222 aa, 25 kDa |
| Observed Molecular Weight | 26-32 kDa |
| GenBank Accession Number | BC032362 |
| Gene Symbol | TREM2 |
| Gene ID (NCBI) | 54209 |
| RRID | AB_3671079 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NZC2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TREM2 (triggering receptor expressed on myeloid cells 2) is a cell surface receptor belongs to TREM family that is expressed on osteoclast, dendritic cells, macrophages, nature killers, neutrophils and microglia (PMID: 19302484). TREM2 is localized predominantly in the Golgi complex, but also shuttles to and from the cell surface in endocytic and exocytic vesicles (PMID: 16675145). TREM2 associates with DAP12 to initiate the intracellular signalling cascade via an immunoreceptor tyrosine-based activation motif (ITAM) domain and tyrosine-kinases (PMID: 23977213).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TREM2 antibody 83438-6-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TREM2 Recombinant monoclonal antibody (240384B7), catalog number 83438-6-RR, has 5 citations published in ACS Nano, Advanced Science, Free Radical Biology and Medicine, Life Sciences, and ACS Infectious Diseases. Research fields span Alzheimer's disease and neural hyperexcitability, prostate cancer progression via macrophage differentiation, sepsis-induced acute lung injury and ferroptosis, circadian rhythm disruption in periodontitis, and mycobacteria–host interactions. Applications cited include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
ACS Nano Modulating Amyloid Pathology-Neural Hyperexcitability Crosstalk for Alzheimer's Disease Therapy. | ||
Adv Sci (Weinh) Cancer Cell-Intrinsic Cholesterol Induces Lipid-Associated Macrophage Differentiation via SP1 Palmitoylation to Promote Prostate Cancer Progression | ||
Free Radic Biol Med TREM2 alleviates sepsis-induced acute lung injury by attenuating ferroptosis via the SHP1/STAT3 pathway | ||
Life Sci Circadian rhythm disruption aggravates periodontitis via orchestrating TREM2+ macrophage-mediated bone resorption.
| ||
ACS Infect Dis A Photoactivatable Free Mycolic Acid Probe to Investigate Mycobacteria-Host Interactions |
Reviews
What customers say
Users report positive results with this antibody for Western Blot at a 1:5000 dilution on microglia, describing it as a great WB antibody. A second user tested it for Immunofluorescence at 1:1000 on iPSC-derived cortical organoids, giving a moderate rating. Overall, the antibody performs well in WB applications, while IF results on organoid samples were less conclusive.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Christin (Verified Customer) (12-22-2025) | Great antibody for western blot!
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Alicia (Verified Customer) (10-07-2025) | Cortical organoid
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