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  • Western Blot (WB) analysis of various lysates using C6orf130 Recombinant antibody (83500-4-RR)

    WB analysis using 83500-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83500-4-RR (C6orf130 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of A431 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of A431 using 83500-4-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Flow cytometry (FC) experiment of PC-3 cells using C6orf130 Recombinant antibody (83500-4-RR)

    FC experiment of PC-3 using 83500-4-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug C6orf130 Recombinant antibody (83500-4-RR, Clone:240455D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

  • Affinity and Kinetic Characterization of 83500-4-RR

    Affinity and Kinetic Characterization of 83500-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83500-4-RR against Human C6orf130 were performed. The affinity constant is below 1 pM.

C6orf130 Recombinant monoclonal antibody

C6orf130 Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83500-4-RR
Clone No.240455D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

OARD1, [Protein ADP-ribosylglutamate] hydrolase OARD1, 240455D7, ADP-ribose glycohydrolase OARD1, EC:3.2.2.-

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inLNCaP cells, HeLa cells, HCT 116 cells
Positive FC (Intra) detected inA431 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83500-4-RR targets C6orf130 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag19341

Product name: Recombinant human C6orf130 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-152 aa of BC011709

Sequence: MASSLNEDPEGSRITYVKGDLFACPKTDSLAHCISEDCRMGAGIAVLFKKKFGGVQELLNQQKKSGEVAVLKRDGRYIYYLITKKRASHKPTYENLQKSLEAMKSHCLKNGVTDLSMPRIGCGLDRLQWENVSAMIEEVFEATDIKITVYTL

Predict reactive species
Full Name chromosome 6 open reading frame 130
Calculated Molecular Weight 152 aa, 17 kDa
Observed Molecular Weight 17 kDa
GenBank Accession NumberBC011709
Gene Symbol C6orf130
Gene ID (NCBI) 221443
RRIDAB_3671126
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y530
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

OARD1 (O-acetyl-ADP-ribose deacetylase 1), also known as TARG1 (Terminal ADP-ribose protein glycohydrolase 1) and C6orf130, is a ADP-ribose glycohydrolase that hydrolyzes ADP-ribose and acts on different substrates, such as proteins ADP-ribosylated on glutamate and O-acetyl-ADP-D-ribose (PMID: 21849506; 23474714; 23481255). The MW of this protein is 17 kDa, and this antibody specially recognises the 17 kDa protein.

Protocols

Product Specific Protocols
FC protocol for C6orf130 antibody 83500-4-RRDownload protocol
WB protocol for C6orf130 antibody 83500-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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