Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells, HEK-293 cells, SH-SY5Y cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HUVEC cells |
| Positive FC (Intra) detected in | A549 cells, HL-60 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1500-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83519-4-RR targets SOD2 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag21388 Product name: Recombinant human SOD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-140 aa of BC016934 Sequence: MLSRAVCGTSRQLAPALGYLGSRQKHSLPDLPYDYGALEPHINAQIMQLHHSKHHAAYVNNLNVTEEKYQEALAKGRFQAERREAVPGRGDPREPGPIRTGLSVEENSLRICTGSEFSRHDSLSFKHMVYLIVEGVPRWV Predict reactive species |
| Full Name | superoxide dismutase 2, mitochondrial |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC016934 |
| Gene Symbol | SOD2 |
| Gene ID (NCBI) | 6648 |
| RRID | AB_3671145 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P04179 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SOD2(superoxide dismutase 2, mitochondrial ) is also named as IPOB, MNSOD, SODM, Mn-SOD and belongs to the iron/manganese superoxide dismutase family. It is a marker of mitochondria, which is restricted to the perinuclear area in a cell with aggregate formation of mutant SOD1(PMID:12659845). It functions as the first line of antioxidant defense against highly reactive superoxide radicals and it appears to be early predictors for survival in septic patients with with MIF(PMID:20863520). It has 2 isoforms with the molecular weight of 25 kDa and 21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for SOD2 antibody 83519-4-RR | Download protocol |
| IF protocol for SOD2 antibody 83519-4-RR | Download protocol |
| IHC protocol for SOD2 antibody 83519-4-RR | Download protocol |
| WB protocol for SOD2 antibody 83519-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SOD2 Recombinant monoclonal antibody (240462H8), catalog number 83519-4-RR, has 4 total citations. These appear in Materials Today Bio, Journal of Ethnopharmacology, Medicine (Baltimore), and bioRxiv. The associated research fields include trigeminal neuralgia treatment via targeted siRNA delivery, diabetic nephropathy and podocyte protection through the NLRP3 inflammasome axis, nitrogen-based flame retardant-induced hepatotoxicity, and quantitative measurement of reactive oxygen species in living cells.
| Species | Application | Title |
|---|---|---|
Mater Today Bio ROS-responsive nanoplatform-mediated targeted intranasal delivery of Piezo2 siRNA for the treatment of trigeminal neuralgia. | ||
J Ethnopharmacol Senkyunolide a from danggui buxue decoction protects podocytes against diabetic nephropathy via the mir-223-3p/nlrp3 inflammasome axis. | ||
Medicine (Baltimore) Network toxicology-driven insights on nitrogen-based flame retardant-induced hepatotoxicity: Computational prediction and experimental validation. | ||
bioRxiv Rosindol: A fluorogen for the quantitative measurement of reactive oxygen species in living cells. |
Reviews
What customers say
Customers rate this antibody highly, with most reviews giving 5 stars. It performs well across multiple applications including Western Blot, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry, and CE-SDS. Users report excellent, specific results with bands matching the manufacturer's reference images. Recommended dilutions range from 1:100 to 1:5000 depending on the application and cell type tested, including corneal cells, ARPE-19, and MIOM1/PBMCs. Overall, reviewers find it reliable and effective for their experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Dhanwini (Verified Customer) (09-24-2025) | GOOD
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Robert (Verified Customer) (09-24-2025) | Used for western and CE-SDS. Expect bands to look similar to the images provided by proteintech.
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Sneha (Verified Customer) (09-24-2025) |
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Michael (Verified Customer) (09-23-2025) | Excellent results from this!
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Henry (Verified Customer) (09-23-2025) | hELPED FOR SPECIFIC RESULTS
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