Tested Applications
| Positive WB detected in | LPS and Brefeldin A treated NR8383 cells |
| Positive IF/ICC detected in | PMA, LPS and Brefeldin A treated THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83758-1-RR targets Cxcl2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | chemokine (C-X-C motif) ligand 2 |
| Calculated Molecular Weight | 11 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | NM_053647.1 |
| Gene Symbol | Cxcl2 |
| Gene ID (NCBI) | 114105 |
| RRID | AB_3671353 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P30348 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CXCL2 is a member of the CXC subfamily, which encodes secreted proteins involved in immunoregulatory and inflammatory processes. CXCL2 is expressed at sites of inflammation and may suppress hematopoietic progenitor cell proliferation. CXCL2 plays an critical role in maintaining cancer-induced macrophage infiltration and the resulting mechanical hypersensitivity and persistent spontaneous nociception (PMID: 36754246).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Cxcl2 antibody 83758-1-RR | Download protocol |
| WB protocol for Cxcl2 antibody 83758-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Cxcl2 Recombinant monoclonal antibody (240367C5), catalog number 83758-1-RR, has 3 total citations. These appear in Frontiers in Immunology, Journal of Inflammation Research, and Chemical Biology & Drug Design. The cited research spans inflammatory and immune-related fields: the IL-1β/CXCL2 axis in periodontitis and inflammatory bowel disease, immune infiltration in alcoholic hepatitis, and NF-κB pathway inhibition in dextran sulfate sodium-induced colitis. Applications include IHC and WB across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Front Immunol Unveiling the IL-1β/CXCL2 axis: a shared therapeutic target in periodontitis and inflammatory bowel disease. | ||
J Inflamm Res Exploration and Validation of Key Genes and Immune Infiltration in Alcoholic Hepatitis. | ||
Chem Biol Drug Des Ginkgetin Attenuates Dextran Sulfate Sodium-Induced Colitis by Inhibiting the Nuclear Factor Kappa B Pathway. |
