Tested Applications
| Positive WB detected in | EL-4 cells, MCF-7 cells, SH-SY5Y cells, T-47D cells |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | Jurkat cells, U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83808-1-RR targets GATA3 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag0174 Product name: Recombinant human GATA3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 224-442 aa of BC003070 Sequence: PYVPEYSSGLFPPSSLLGGSPTGFGCKSRPKARSSTGRECVNCGATSTPLWRRDGTGHYLCNACGLYHKMNGQNRPLIKPKRRLSAARRAGTSCANCQTTTTTLWRRNANGDPVCNACGLYYKLHNINRPLTMKKEGIQTRNRKMSSKSKKCKKVHDSLEDFPKNSSFNPAALSRHMSSLSHISPFSHSSHMLTTPTPMHPPSSLSFGPHHPSSMVTAM Predict reactive species |
| Full Name | GATA binding protein 3 |
| Calculated Molecular Weight | 443 aa, 48 kDa |
| Observed Molecular Weight | 37 kDa and 48 kDa |
| GenBank Accession Number | BC003070 |
| Gene Symbol | GATA3 |
| Gene ID (NCBI) | 2625 |
| RRID | AB_3671395 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | P23771 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for GATA3 antibody 83808-1-RR | Download protocol |
| IF protocol for GATA3 antibody 83808-1-RR | Download protocol |
| WB protocol for GATA3 antibody 83808-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
