GATA3 Recombinant antibody, PBS Only (Capture)

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  WB analysis using 83808-1-RR (same clone as 83808-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 83808-1-RR (same clone as 83808-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 83808-1-RR (GATA3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP01933-1

  Sandwich ELISA standard curve of MP01933-1, Human GATA3 Recombinant Matched Antibody Pair - PBS only. 83808-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag0174. 83808-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  Sample test of MP01933-1

  The mean GATA3 concentration was determined to be 21.82 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

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  FC experiment of Jurkat using 83808-1-RR (same clone as 83808-1-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  FC experiment of U937 using 83808-1-RR (same clone as 83808-1-PBS)

  1x10^6 U937 were intracellularly stained with 0.25 ug GATA3 Recombinant antibody (83808-1-RR, Clone:240684A2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83808-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01933-1

  Cytometric bead array standard curve of MP01933-1, GATA3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83808-1-PBS. Detection antibody: 83808-4-PBS. Standard: Ag0174. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83808-1-RR

  Biolayer interferometry (BLl) kinetic assays of 83808-1-RR against Human GATA3 were performed. The affinity constant is 0.686 nM.

GATA3 Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 83808-1-PBS

Clone No.240684A2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Trans-acting T-cell-specific transcription factor GATA-3, GATA-binding factor 3, GATA binding factor 3, 240684A2

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Product Information

83808-1-PBS targets GATA3 as part of a matched antibody pair:

MP01933-1: 83808-1-PBS capture and 83808-4-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: GATA3 fusion protein Ag0174
• Full Name: GATA binding protein 3
• Calculated Molecular Weight: 443 aa, 48 kDa
• Observed Molecular Weight: 37 kDa and 48 kDa
• GenBank Accession Number: BC003070
• Gene Symbol: GATA3
• Gene ID (NCBI): 2625
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P23771
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Trans-acting T-cell-specific transcription factor, also known as GATA3, belongs to the zinc-finger transcription factor GATA family which drives the differentiation of T helper (Th) 2 cells. GATA factors are expressed in a tissue-specific manner and GATA3 is present in both hematopoietic (e.g., T cells) and non-hematopoietic tissues, including the kidney, central nervous system, skin, and mammary gland (PMID: 25148023). GATA3 plays a central role in Th2 responses both in vitro and in vivo (PMID: 16467870). The full-length GATA3 protein contains 444 aa corresponding to the molecular weights of 48 kDa, and the truncated protein was detected at approximately 37 kDa (PMID: 24758297).