Tested Applications
| Positive WB detected in | pervanadate treated HeLa cells |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:125-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83933-1-RR targets Phospho-FAK (Tyr397) in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | PTK2 protein tyrosine kinase 2 |
| Calculated Molecular Weight | 1052 aa, 119 kDa |
| Observed Molecular Weight | 110 kDa |
| GenBank Accession Number | BC028733 |
| Gene Symbol | FAK |
| Gene ID (NCBI) | 5747 |
| RRID | AB_3671514 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q05397 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FAK (Focal adhesion kinase 1) is also named as FAK1, FADK, pp125FAK, FAK and belongs to the protein kinase superfamily. It is a critical tyrosine kinase that modulates cell adhesion, migration, proliferation and survival in response to extracellular signals (PMID:19664602). It also acts as a pivotal signal 'integrator', controlling and coordinating cellular responses that include cell migration, survival, proliferation and, epithelial tissue repair after DNA damage (PMID:20966971). This protein has some isoforms produced by alternative splicing and contains several phosphorylation and SUMOylation modification sites.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Phospho-FAK (Tyr397) antibody 83933-1-RR | Download protocol |
| WB protocol for Phospho-FAK (Tyr397) antibody 83933-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Phospho-FAK (Tyr397) Recombinant monoclonal antibody (240630F8), catalog number 83933-1-RR, has 25 citations. These appear in journals including Nature Communications, Bioactive Materials, Cardiovascular Diabetology, Journal of Medicinal Chemistry, European Journal of Medicinal Chemistry, Biochemical Pharmacology, International Journal of Molecular Sciences, iScience, American Journal of Pathology, Cancer Science, PLoS One, and British Journal of Pharmacology, among others. Research fields span cancer biology (pancreatic, gastric, lung, breast, osteosarcoma), wound healing, diabetic kidney disease, fibrosis, metastasis, and cell adhesion signaling.
| Species | Application | Title |
|---|---|---|
Bioact Mater Chondrogenic niche hydrogel microspheres facilitate cartilage regeneration in osteoarthritis. | ||
Cardiovasc Diabetol Targeting TFAM K76 acetylation attenuates mitochondrial dysfunction and kidney injury in diabetic kidney disease. | ||
Adv Healthc Mater Conserved Sequence-Derived from Keratins Guides Design of Self-Assembling Protein for Accelerated Wound Healing. | ||
J Med Chem Cyclic Heptapeptide FZ1 Acts as an Integrin αvβ3 Agonist to Facilitate Diabetic Skin Wound Healing by Enhancing Angiogenesis. | ||
Eur J Med Chem Discovery of a potent anticancer agent against pancreatic ductal adenocarcinoma targeting FAK with DFG-out state and JAK/Aurora kinases | ||
Biochem Pharmacol ICAP-1 alternative splicing regulates Talin tension polarization in NSCLC durotaxis. |
Reviews
What customers say
The single review for this product is highly positive, with a 5-star rating. The reviewer used the antibody on MSCs for both Western Blot (1:1000) and Immunofluorescence (1:300), reporting that it "worked well" in both applications. Overall, the feedback indicates reliable performance across multiple techniques.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Udesh (Verified Customer) (08-12-2025) | Worked well for both IF and WB
|
