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  • Western Blot (WB) analysis of various lysates using TIMP2 Recombinant antibody (83938-3-RR)

    WB analysis using 83938-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83938-3-RR (TIMP2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

TIMP2 Recombinant antibody

TIMP2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83938-3-RR
Clone No.243160E10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

TIMP-2, CSC 21K, CSC-21K, Metalloproteinase inhibitor 2, TIMP 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inA549 cells, HT-1080 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83938-3-RR targets TIMP2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TIMP2 fusion protein Eg0430 Predict reactive species
Full Name TIMP metallopeptidase inhibitor 2
Calculated Molecular Weight 220 aa, 24 kDa
Observed Molecular Weight 21-24 kDa
GenBank Accession NumberBC052605
Gene Symbol TIMP2
Gene ID (NCBI) 7077
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP16035
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The TIMPs are natural highly specific endogenous inhibitors of MMPs. Human TIMP family consists of four 21-28 kDa proteins known as TIMP1, TIMP2, TIMP3, and TIMP4 encoded by four paralogous genes.TIMPs are vital to the maintenance of ECM homeostasis primarily through their MMP inhibitory functions. TIMP2, a member of the TIMP family, regulates the proteolytic activity of all MMPs and is involved in cell differentiation, growth, migration, angiogenesis, and apoptosis. Urinary TIMP2 has been recently recognized as an early biomarker to predict Acute kidney injury (AKI) in critically ill patients.

Protocols

Product Specific Protocols
WB protocol for TIMP2 antibody 83938-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols