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  • Western Blot (WB) analysis of human saliva using SLPI Recombinant antibody (83965-4-RR)

    WB analysis of human saliva using 83965-4-RR

    human saliva were subjected to SDS PAGE followed by western blot with 83965-4-RR (SLPI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using SLPI Recombinant antibody (83965-4-RR)

    WB analysis of PC-3 using 83965-4-RR

    WB result of SLPI antibody (83965-4-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SLPI transfected PC-3 cells.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SLPI Recombinant antibody (83965-4-RR)

    IHC staining of human cervical cancer using 83965-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83965-4-RR (SLPI antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using SLPI Recombinant antibody (83965-4-RR)

    IHC staining of human cervical cancer using 83965-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 83965-4-RR (SLPI antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83965-4-RR

    Affinity and Kinetic Characterization of 83965-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83965-4-RR against Human SLPI were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO Validated

SLPI Recombinant monoclonal antibody

SLPI Uni-rAb® Recombinant Antibody for WB, IHC, ELISA
Cat No. 83965-4-RR
Clone No.241057F8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

241057F8, ALK1, ALP, Antileukoprotease, Antileukoproteinase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman saliva, PC-3 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83965-4-RR targets SLPI in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag1673

Product name: Recombinant human SLPI protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-132 aa of BC020708

Sequence: MKSSGLFPFLVLLALGTLAPWAVEGSGKSFKAGVCPPKKSAQCLRYKKPECQSDWQCPGKKRCCPDTCGIKCLDPVDTPNPTRRKPGKCPVTYGQCLMLNPPNFCEMDGQCKRDLKCCMGMCGKSCVSPVKA

Predict reactive species
Full Name secretory leukocyte peptidase inhibitor
Calculated Molecular Weight 14 kDa
Observed Molecular Weight14 kDa
GenBank Accession NumberBC020708
Gene Symbol SLPI
Gene ID (NCBI) 6590
RRIDAB_3671543
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP03973
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLPI, a member of Whey acidic protein family, could mediate a variety of biological processes including cell proliferation and apoptosis, repairing reaction and immune response, with crucial roles in anti-protease, anti-inflammatory, anti-bacterial and anti-virus. SLPI is mainly expressed in epithelial cells.

Protocols

Product Specific Protocols
IHC protocol for SLPI antibody 83965-4-RRDownload protocol
WB protocol for SLPI antibody 83965-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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