Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells, mouse brain tissue, rat brain tissue, mouse spleen tissue, mouse cerebellum tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84068-4-RR targets CRTAM/CD355 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Eg1541 Product name: Recombinant Human CRTAM protein (His Tag) Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 18-286 aa of NM_019604.4 Sequence: SLTNHTETITVEEGQTLTLKCVTSLRKNSSLQWLTPSGFTIFLNEYPALKNSKYQLLHHSANQLSITVPNVTLQDEGVYKCLHYSDSVSTKEVKVIVLATPFKPILEASVIRKQNGEEHVVLMCSTMRSKPPPQITWLLGNSMEVSGGTLHEFETDGKKCNTTSTLIIHTYGKNSTVDCIIRHRGLQGRKLVAPFRFEDLVTDEETASDALERNSLSSQDPQQPTSTVSVTEDSSTSEIDKEEKEQTTQDPDLTTEANPQYLGLARKKS Predict reactive species |
| Full Name | cytotoxic and regulatory T cell molecule |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | NM_019604.4 |
| Gene Symbol | CRTAM |
| Gene ID (NCBI) | 56253 |
| RRID | AB_3671636 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O95727-1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Cytotoxic and regulatory T cell molecule (CRTAM, also known as CD355) is an immunoglobulin-superfamily transmembrane protein with V and C1-like Ig domains, which is involved in epithelial cell adhesion (PMID: 18329370; 20556794). CRTAM is critical to instruct the differentiation of CD4+ cytotoxic T cells (CTL) through the induction of eomesodermin and CTL-related genes (PMID: 26694968). It also regulates CD8+ T cell retention within the lymph node and eventually effector function (PMID: 19752223). The predicted molecular weight of CRTAM is 43 kDa. Due to glycosylation, the observed molecular weight of CRTAM can be apparent at ~80 kDa (PMID: 16300832).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CRTAM/CD355 antibody 84068-4-RR | Download protocol |
| IHC protocol for CRTAM/CD355 antibody 84068-4-RR | Download protocol |
| WB protocol for CRTAM/CD355 antibody 84068-4-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CRTAM/CD355 Recombinant monoclonal antibody (catalog number 84068-4-RR) has 1 total citation. It appears in the journal Cancer Immunology & Immunotherapy (2025), in a study titled "Tumor-draining lymph nodes respond to immune checkpoint inhibition and orchestrate tumor immune remodeling." The associated research field is cancer immunology, specifically tumor immune remodeling and immune checkpoint inhibition, with the antibody applied to human samples via IHC.
| Species | Application | Title |
|---|---|---|
Cancer Immunol Immunother Tumor-draining lymph nodes respond to immune checkpoint inhibition and orchestrate tumor immune remodeling. |
