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  • Sandwich ELISA standard curve of MP01071-4

    Sandwich ELISA standard curve of MP01071-4

    Sandwich ELISA standard curve of MP01071-4, Human PCSK9 Recombinant Matched Antibody Pair - PBS only. 84172-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0362. 84172-6-PBS was HRP conjugated as the detection antibody. Range: 195-6250 pg/mL.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    Plasma of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 206.0 ng/mL with a range of ND-514.5 ng/mL.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    HepG2 were cultured in DMEM supplemented with 10% fetal bovine serum, 2.5 mM L-glutamine, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. An aliquot of the cell culture supernate was removed, assayed for human PCSK9, and measured 81.6 ng/mL.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    The mean PCSK9 concentration was determined to be 6.6 ng/mL in HepG2 cell extract based on a 2.0 mg/mL extract load.

  • Sample test of MP01071-4

    Sample test of MP01071-4

    Serum of six individual healthy human donors was measured. The PCSK9 concentration of detected samples was determined to be 120.8 ng/mL with a range of ND-421.1 ng/mL.

  • Affinity and Kinetic Characterization of 84172-6-PBS

    Affinity and Kinetic Characterization of 84172-6-PBS

    Biolayer interferometry (BLl) kinetic assay of 84172-6-PBS against Human PCSK9 was performed. The affinity constant is 2.55 nM.

PCSK9 Recombinant monoclonal antibody, PBS Only (Detector)

PCSK9 Uni-rAb® Recombinant Antibody for Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84172-6-PBS
Clone No.241395B7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

EC:3.4.21.-, FH3, HCHOLA3, LDLCQ1, NARC 1

Formulation:  PBS Only
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Product Information

84172-6-PBS targets PCSK9 as part of a matched antibody pair:

MP01071-4: 84172-4-PBS capture and 84172-6-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0362

Product name: Recombinant Human PCSK9 protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: C-6*HIS

Domain: 31-692 aa of NM_174936.4

Sequence: QEDEDGDYEELVLALRSEEDGLAEAPEHGTTATFHRCAKDPWRLPGTYVVVLKEETHLSQSERTARRLQAQAARRGYLTKILHVFHGLLPGFLVKMSGDLLELALKLPHVDYIEEDSSVFAQSIPWNLERITPPRYRADEYQPPDGGSLVEVYLLDTSIQSDHREIEGRVMVTDFENVPEEDGTRFHRQASKCDSHGTHLAGVVSGRDAGVAKGASMRSLRVLNCQGKGTVSGTLIGLEFIRKSQLVQPVGPLVVLLPLAGGYSRVLNAACQRLARAGVVLVTAAGNFRDDACLYSPASAPEVITVGATNAQDQPVTLGTLGTNFGRCVDLFAPGEDIIGASSYCSTCFVSQSGTSQAAAHVAGIAAMMLSAEPELTLAELRQRLIHFSAKDVINEAWFPEDQRVLTPNLVAALPPSTHGAGWQLFCRTVWSAHSGPTRMATAVARCAPDEELLSCSSFSRSGKRRGERMEAQGGKLVCRAHNAFGGEGVYAIARCCLLPQANCSVHTAPPAEASMGTRVHCHQQGHVLTGCSSHWEVEDLGTHKPPVLRPRGQPNQCVGHREASIHASCCHAPGLECKVKEHGIPAPQEQVTVACEEGWTLTGCSALPGTSHVLGAYAVDNTCVVRSRDVSTTGSTSEGAVTAVAICCRSRHLAQASQELQ

Predict reactive species
Full Name proprotein convertase subtilisin/kexin type 9
Calculated Molecular Weight74 kDa
GenBank Accession NumberNM_174936.4
Gene Symbol PCSK9
Gene ID (NCBI) 255738
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ8NBP7
Storage Buffer PBS only, pH 7.3.
Storage ConditionsStore at -80°C.
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