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  • Western Blot (WB) analysis of various lysates using MED6 Recombinant antibody (84184-4-RR)

    WB analysis using 84184-4-RR (same clone as 84184-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84184-4-RR (MED6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01087-3

    Sandwich ELISA standard curve of MP01087-3

    Sandwich ELISA standard curve of MP01087-3, Human MED6 Recombinant Matched Antibody Pair - PBS only. 84184-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag7541. 84184-2-PBS was HRP conjugated as the detection antibody. Range: 156-5000 pg/mL

  • Sample test of MP01087-3

    Sample test of MP01087-3

    The mean MED6 concentration was determined to be 7.45 ng/mL in HEK-293 cell extract based on a 1.5 mg/mL extract load, 16.00 ng/mL in Jurkat cell extract based on a 1.5 mg/mL extract load and 13.01 ng/mL in U2OS cell extract based on a 3.1 mg/mL extract load.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using MED6 Recombinant antibody (84184-4-RR)

    IF Staining of A431 using 84184-4-RR (same clone as 84184-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using MED6 antibody (84184-4-RR, Clone: 241382H2 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using MED6 Recombinant antibody (84184-4-RR)

    FC experiment of A431 using 84184-4-RR (same clone as 84184-4-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug MED6 Recombinant antibody (84184-4-RR, Clone:241382H2) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84184-4-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01087-3

    Cytometric bead array standard curve of MP01087-3

    Cytometric bead array standard curve of MP01087-3, MED6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84184-4-PBS. Detection antibody: 84184-2-PBS. Standard: Ag7541. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 84184-4-RR

    Affinity and Kinetic Characterization of 84184-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84184-4-RR against Human MED6 were performed. The affinity constant is below 1 pM.

MED6 Recombinant antibody, PBS Only (Capture)

MED6 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 84184-4-PBS
Clone No.241382H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241382H2, Activator-recruited cofactor 33 kDa component, ARC33, hMed6, Mediator of RNA polymerase II transcription subunit 6

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Product Information

84184-4-PBS targets MED6 as part of a matched antibody pair:

MP01087-3: 84184-4-PBS capture and 84184-2-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen MED6 fusion protein Ag7541 Predict reactive species
Full Name mediator complex subunit 6
Calculated Molecular Weight 28 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberBC004106
Gene Symbol MED6
Gene ID (NCBI) 10001
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75586
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

MED6 is a part of mediator complex. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.