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MLPH Recombinant monoclonal antibody, PBS Only (Capture) (84282-1-PBS)

Filter: AllWBSandwich ELISASample testIHCAffinity

WB analysis using 84282-1-RR (same clone as 84282-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84282-1-RR (MLPH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP01196-3
Sandwich ELISA standard curve of MP01196-3, Human MLPH Recombinant Matched Antibody Pair - PBS only. 84282-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag32931. 84282-4-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.
Sample test of MP01196-3
The mean MLPH concentration was determined to be 5.9 ng/mL in A549 cell extract based on a 1.5 mg/mL extract load and 1.7 ng/mL in HT-29 cell extract based on a 1.5 mg/mL extract load.
IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.
IHC staining of human skin cancer using 84282-1-RR (same clone as 84282-1-PBS)
Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 84282-1-RR (MLPH antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84282-1-PBS in a different storage buffer formulation.
Affinity and Kinetic Characterization of 84282-1-RR
Biolayer interferometry (BLl) kinetic assays of 84282-1-RR against Human MLPH were performed. The affinity constant is 2.43 nM.

MLPH Recombinant monoclonal antibody, PBS Only (Capture)

Name: MLPH Recombinant monoclonal antibody, PBS Only (Capture)
Brand: Proteintech
SKU: 84282-1-PBS

MLPH Uni-rAb^® Recombinant Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84282-1-PBS

Clone No.241621G4

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

241621G4, Exophilin 3, Exophilin-3, Melanophilin, SLAC2 A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84282-1-PBS targets MLPH as part of a matched antibody pair:

MP01196-3: 84282-1-PBS capture and 84282-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag32931 Product name: Recombinant human MLPH protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 372-600 aa of BC001653 Sequence: AGVRTEADVEEEALRRKLEELTSNVSDQETSSEEEEAKDEKAEPNRDKSVGPLPQADPEVGTAAHQTNRQEKSPQDPGDPVQYNRTTDEELSELEDRVAVTASEVQQAESEVSDIESRIAALRAAGLTVKPSGKPRRKSNLPIFLPRVAGKLGKRPEDPNADPSSEAKAMAVPYLLRRKFSNSLKSQGKDDDSFDRKSVYRGSLTQRNPNARKGMASHTFAKPVVAHQS Predict reactive species
Full Name	melanophilin
Calculated Molecular Weight	66 kDa
Observed Molecular Weight	66 kDa, 80 kDa
GenBank Accession Number	BC001653
Gene Symbol	MLPH
Gene ID (NCBI)	79083
RRID	AB_3671828
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9BV36
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Melanophilin (Mlph) is a member of the exophilin subfamily of Rab effector proteins. MLPH is a modular protein, forming a ternary complex with the small Ras-related GTPase Rab27A in its GTP-bound form and the motor protein myosin Va. It regulates retention of melanosomes at the peripheral actin cytoskeleton of melanocytes, a process essential for normal mammalian pigmentation. Defects in MLPH are a cause of Griscelli syndrome type 3 (GS3), which is characterized by a silver-gray hair color and abnormal pigment distribution in the hair shaft.