Filter: AllWBSandwich ELISASample testIHCIF/ICCCytometric bead array

WB analysis using 84430-2-RR (same clone as 84430-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.
WB analysis using 84430-2-RR (same clone as 84430-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP01311-4
Sandwich ELISA standard curve of MP01311-4, Mouse SIRP alpha/CD172a Recombinant Matched Antibody Pair - PBS only. 84430-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1520. 84430-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.
Sample test of MP01311-4
The mean SIRP alpha/CD172a concentration was determined to be 1,352.0 ng/mL in mouse brain tissue extract based on a 2.0 mg/mL extract load, 1,425.8 ng/mL in mouse cerebellum tissue extract based on a 2.9 mg/mL extract load, 796.6 ng/mL in RAW 264.7 cell extract based on a 4.2 mg/mL extract load and 4.0 ng/mL in NIH/3T3 cell extract based on a 4.4 mg/mL extract load.
Sample test of MP01311-4
The mean SIRP alpha/CD172a concentration was determined to be 595.2 ng/mL in mouse brain tissue homogenate based on a 2.2 mg/mL extract load.
Sample test of MP01311-4
Serum of six mice was measured. The SIRP alpha/CD172a concentration of detected samples was determined to be 69.0 ng/mL with a range of 59.9-73.9 ng/mL.
IHC staining of mouse brain using 84430-2-RR (same clone as 84430-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 84430-2-RR (same clone as 84430-2-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.
IF Staining of RAW 264.7 using 84430-2-RR (same clone as 84430-2-PBS)
Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SIRP alpha/CD172a antibody (84430-2-RR, Clone: 241794D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84430-2-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01311-2
Cytometric bead array standard curve of MP01311-2, MOUSE SIRP Alpha/CD172a Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84430-2-PBS. Detection antibody: 84430-3-PBS. Standard: Eg1520. Range: 0.781-100 ng/mL.

Mouse SIRP Alpha/CD172a Recombinant antibody, PBS Only (Capture/Detector)

SIRP Alpha/CD172a Uni-rAb^TM Recombinant Antibody for WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84430-2-PBS

Clone No.241794D9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs, Bit

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84430-2-PBS targets SIRP Alpha/CD172a as part of a matched antibody pair:

MP01311-2: 84430-2-PBS capture and 84430-3-PBS detection (validated in Cytometric bead array)

MP01311-4: 84430-3-PBS capture and 84430-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	FusionProtein Predict reactive species
Full Name	signal-regulatory protein alpha
Calculated Molecular Weight	56 kDa
Observed Molecular Weight	90-140 kDa
GenBank Accession Number	NM_007547.4
Gene Symbol	Sirpa
Gene ID (NCBI)	19261
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P97797-2
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).