SIRP Alpha/CD172a Recombinant antibody

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  WB analysis using 84430-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 84430-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84430-2-RR (SIRP alpha/CD172a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84430-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84430-2-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84430-2-RR (SIRP Alpha/CD172a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of RAW 264.7 using 84430-2-RR

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using SIRP alpha/CD172a antibody (84430-2-RR, Clone: 241794D9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

SIRP Alpha/CD172a {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 84430-2-RR

Clone No.241794D9

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Inhibitory receptor SHPS-1, CD172a, CD172 antigen-like family member A, Brain Ig-like molecule with tyrosine-based activation motifs, Bit

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Tested Applications

• Positive WB detected in: mouse brain tissue, RAW 264.7 cells
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84430-2-RR targets SIRP Alpha/CD172a in WB, IHC, IF/ICC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: FusionProtein
• Full Name: signal-regulatory protein alpha
• Calculated Molecular Weight: 56 kDa
• Observed Molecular Weight: 90-140 kDa
• GenBank Accession Number: NM_007547.4
• Gene Symbol: Sirpa
• Gene ID (NCBI): 19261
• RRID: AB_3671957
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P97797-2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SIRP Alpha, also known as CD172a, SHPS-1, and BIT, belongs to the SIRP family. SIRP Alpha is a transmembrane glycoprotein expressed explicitly on myeloid cells and provides a "do not eat me" signal after engaging with integrin-associated protein CD47 on tumor cells (PMID: 36419386). SIRP Alpha shows heterogeneity in molecular weight (~65-120 kDa) in various tissues due to differential glycosylation (PMID: 18051954).