• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of various lysates using CKAP4 Recombinant antibody (84485-5-RR)

    WB analysis using 84485-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84485-5-RR (CKAP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CKAP4 Recombinant antibody (84485-5-RR)

    IHC staining of mouse brain using 84485-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84485-5-RR (CKAP4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CKAP4 Recombinant antibody (84485-5-RR)

    IHC staining of mouse brain using 84485-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84485-5-RR (CKAP4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 84485-5-RR

    Affinity and Kinetic Characterization of 84485-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84485-5-RR against Human CKAP4 were performed. The affinity constant is 15.1 nM.

CKAP4 Recombinant antibody

CKAP4 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84485-5-RR
Clone No.241871C5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

241871C5, 63 kDa membrane protein, CLIMP 63, Climp-63, ERGIC 63

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inA431 cells, HeLa cells, A549 cells, Caco-2 cells, NIH/3T3 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84485-5-RR targets CKAP4 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CKAP4 fusion protein Ag10022 Predict reactive species
Full Name cytoskeleton-associated protein 4
Calculated Molecular Weight 602 aa, 66 kDa
Observed Molecular Weight 63 kDa
GenBank Accession NumberBC082972
Gene Symbol CKAP4
Gene ID (NCBI) 10970
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ07065
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CKAP4 (Cytoskeleton-associated protein 4), also known as CLIMP-63 (cytoskeleton-linking membrane protein 63), is a 63 kDa cytoskeleton-linking membrane protein. CKAP4 is a novel DKK1-binding protein on the cell surface membrane of Madin-Darby canine kidney (MDCK) epithelial cells, and evaluated CKAP4-mediated DKK1 signaling in cancer cell proliferation (PMID: 27322059). In addition, CKAP4 was modified with palmitate at Cys100 by palmitoyl acyltransferase DHHC2 (PMID: 18296695), and palmitoylation was required for CKAP4 localization to PM lipid rafts to promote cancer cell proliferation (PMID: 31744930).

Protocols

Product Specific Protocols
WB protocol for CKAP4 antibody 84485-5-RRDownload protocol
IHC protocol for CKAP4 antibody 84485-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols