Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells, HeLa cells, MDA-MB-231 cells, T-47D cells |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
84537-1-RR targets RAD51C in WB, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag35070 Product name: Recombinant human RAD51C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-376 aa of BC107753 Sequence: MRGKTFRFEMQRDLVSFPLSPAVRVKLVSAGFQTAEELLEVKPSELSKEVGISKAEALETLQIIRRECLTNKPRYAGTSESHKKCTALELLEQEHTQGFIITFCSALDDILGGGVPLMKTTEICGAPGVGKTQLCMQLAVDVQIPECFGGVAGEAVFIDTEGSFMVDRVVDLATACIQHLQLIAEKHKGEEHRKALEDFTLDNILSHIYYFRCRDYTELLAQVYLLPDFLSEHSKVRLVIVDGIAFPFRHDLDDLSLRTRLLNGLAQQMISLANNHRLAVILTNQMTTKIDRNQALLVPALGESWGHAATIRLIFHWDRKQRLATLYKSPSQKECTVLFQIKPQGFRDTVVTSACSLQTEGSLSTRKRSRDPEEEL Predict reactive species |
| Full Name | RAD51 homolog C (S. cerevisiae) |
| Calculated Molecular Weight | 376 aa, 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | BC107753 |
| Gene Symbol | RAD51C |
| Gene ID (NCBI) | 5889 |
| RRID | AB_3672044 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O43502 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RAD51C (DNA repair protein RAD51 homolog 3) is essential for the homologous recombination (HR) pathway of DNA repair and is involved in the homologous recombination repair (HRR) pathway of double-stranded DNA breaks arising during DNA replication or induced by DNA-damaging agents (PMID: 14716019; 16215984; 16395335).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for RAD51C antibody 84537-1-RR | Download protocol |
| WB protocol for RAD51C antibody 84537-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RAD51C Recombinant monoclonal antibody (241971D4), catalog number 84537-1-RR, has a total of 1 citation. The citation appears in the International Journal of Surgery (Int J Surg). The associated research field is bladder cancer, specifically investigating RAD51C as a hub gene and potential therapeutic target through bioinformatics and experimental analysis.
| Species | Application | Title |
|---|---|---|
Int J Surg Potential of RAD51C as a hub gene and therapeutic target in bladder cancer: insights from bioinformatics and experimental analysis. |
Reviews
What customers say
One reviewer (Jehan Mody) rated this antibody 5/5 stars for Western Blot on OVCAR8 cells at a 1:1000 dilution in 5% milk/TBST. They reported that the antibody produced a clear band at the correct molecular weight for the target protein. Overall, the feedback is positive, confirming reliable specificity and performance under standard WB conditions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Jehan (Verified Customer) (05-27-2025) | Antibody produced a clear band at the right size for the desired protein
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