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  • Western Blot (WB) analysis of HepG2 cells using TMEM175 Recombinant antibody (84554-1-RR)

    WB analysis of HepG2 using 84554-1-RR

    WB result of TMEM175 antibody (84554-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TMEM175 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using TMEM175 Recombinant antibody (84554-1-RR)

    WB analysis using 84554-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84554-1-RR (TMEM175 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84554-1-RR

    Affinity and Kinetic Characterization of 84554-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84554-1-RR against Human TMEM175 were performed. The affinity constant is 5.17 nM.

  • Featured Product
  • KD/KO Validated

TMEM175 Recombinant antibody

TMEM175 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 84554-1-RR
Clone No.241567B9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

241567B9, hTMEM175, Transmembrane protein 175

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84554-1-RR targets TMEM175 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TMEM175 fusion protein Ag13890 Predict reactive species
Full Name transmembrane protein 175
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight56 kDa
GenBank Accession NumberBC005158
Gene Symbol TMEM175
Gene ID (NCBI) 84286
RRIDAB_3672062
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9BSA9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TMEM175 has two repeats of 6-transmembrane-spanning segments and has no GYG K+ channel sequence signature-containing, pore-forming P loop. Lysosomes lacking TMEM175 exhibit no K+conductance, have a markedly depolarized ΔΨ and little sensitivity to changes in [K+], and have compromised luminal pH stability and abnormal fusion with autophagosomes during autophagy. TMEM175 comprises a K+ channel that underlies the molecular mechanism of lysosomal K+ permeability. It has two isoforms with MW 41-45 kDa and 54-60 kDa. For optimal WB detection with this antibody, we recommend avoiding boiling the sample after lysis.

Protocols

Product Specific Protocols
WB protocol for TMEM175 antibody 84554-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols