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  • Western Blot (WB) analysis of various lysates using CTRL Recombinant antibody (84572-4-RR)

    WB analysis using 84572-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84572-4-RR (CTRL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse pancreas tissue using CTRL Recombinant antibody (84572-4-RR)

    WB analysis of mouse pancreas using 84572-4-RR

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 84572-4-RR (CTRL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CTRL Recombinant antibody (84572-4-RR)

    IHC staining of mouse pancreas using 84572-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84572-4-RR (CTRL antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CTRL Recombinant antibody (84572-4-RR)

    IHC staining of mouse pancreas using 84572-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84572-4-RR (CTRL antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CTRL Recombinant antibody

CTRL Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 84572-4-RR
Clone No.241858D7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

241858D7, chymotrypsin like, Chymotrypsin-like protease CTRL-1, CTRL1, EC:3.4.21.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inmouse stomach tissue, mouse pancreas tissue, rat pancreas tissue
Positive IHC detected inmouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84572-4-RR targets CTRL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CTRL fusion protein Eg2214 Predict reactive species
Full Name chymotrypsin-like
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC063475
Gene Symbol CTRL
Gene ID (NCBI) 1506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP40313
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CTRL(Chymotrypsin-like protease) is also named as CTRL1 and belongs to the peptidase S1 family. The CTRL1 enzyme is active over a broad pH range. It expresses in pancreas and presence in intestinal fluid after stimulation of pancreatic secretion as a digestive enzyme(PMID:9065485). This gene encode a protein of 264 amino acid and the protein has a signal peptide of 18 amino acid and a propeptide of 15 amino acid.

Protocols

Product Specific Protocols
WB protocol for CTRL antibody 84572-4-RRDownload protocol
IHC protocol for CTRL antibody 84572-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols