Filter: AllWBSandwich ELISASample testIF/ICCCytometric bead arrayAffinity

WB analysis using 84610-1-RR (same clone as 84610-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84610-1-RR (INTS6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84610-1-PBS in a different storage buffer formulation.
Sandwich ELISA standard curve of MP01446-1
Sandwich ELISA standard curve of MP01446-1, Human INTS6 Recombinant Matched Antibody Pair - PBS only. 84610-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag36146. 84610-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL.
Sample test of MP01446-1
The mean INTS6 concentration was determined to be 61.44 ng/mL in DU 145 cell extract based on a 1.00 mg/mL extract load.
IF Staining of HeLa using 84610-1-RR (same clone as 84610-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84610-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 84610-1-RR (same clone as 84610-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using INTS6 antibody (84610-1-RR, Clone: 242099C7 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84610-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01446-1
Cytometric bead array standard curve of MP01446-1, INTS6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84610-3-PBS. Detection antibody: 84610-1-PBS. Standard: Ag36146. Range: 0.313-40 ng/mL.
Affinity and Kinetic Characterization of 84610-1-PBS
Biolayer interferometry (BLl) kinetic assays of 84610-1-PBS against Human INTS6 were performed. The affinity constant is 0.63 nM.
Affinity and Kinetic Characterization of 84610-1-RR
Biolayer interferometry (BLl) kinetic assays of 84610-1-RR against Human INTS6 were performed. The affinity constant is 0.63 nM.

INTS6 Recombinant monoclonal antibody, PBS Only (Detector)

INTS6 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Cat No. 84610-1-PBS

Clone No.242099C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Synonyms

242099C7, DBI 1, DBI1, DDX26, DDX26A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

Print datasheet

MSDS SDS

Product Information

84610-1-PBS targets INTS6 as part of a matched antibody pair:

MP01446-1: 84610-3-PBS capture and 84610-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag36146 Product name: Recombinant human INTS6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 672-815 aa of BC039829 Sequence: VVNNHIGGKGPPAPTTQAQPDLIKPLPLHKISETTNDSIIHDVVENHVADQLSSDITPNAMDTEFSASSPASLLERPTNHMEALGHDHLGTNDLTVGGFLENHEEPRDKEQCAEENIPASSLNKGKKLMHCRSHEEVNTELKAQ Predict reactive species
Full Name	integrator complex subunit 6
Calculated Molecular Weight	100 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC039829
Gene Symbol	INTS6
Gene ID (NCBI)	26512
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9UL03
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

INTS6 is a component of the Integrator (INT) complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing.