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  • Western Blot (WB) analysis of various lysates using Fc epsilon RI alpha Recombinant antibody (84662-3-RR)

    WB analysis using 84662-3-RR

    Human testis was subjected to SDS PAGE followed by western blot with 84662-3-RR (Fc epsilon RI alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FceR1a Recombinant antibody

FceR1a {ptg:Brand} Recombinant Antibody for WB, ELISA
Cat No. 84662-3-RR
Clone No.241848D1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A, Fc epsilon RI alpha

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inhuman testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84662-3-RR targets FceR1a in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
Calculated Molecular Weight30kDa
Observed Molecular Weight55-65 kDa
GenBank Accession NumberNM_001387280.1
Gene Symbol FceR1a
Gene ID (NCBI) 2205
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12319
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).

Protocols

Product Specific Protocols
WB protocol for FceR1a antibody 84662-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols