FceR1a Recombinant monoclonal antibody, PBS Only

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  WB analysis using 84662-3-RR (same clone as 84662-3-PBS)

  Human testis was subjected to SDS PAGE followed by western blot with 84662-3-RR (Fc epsilon RI alpha antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84662-3-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84662-3-PBS

  Biolayer interferometry (BLl) kinetic assays of 84662-3-PBS against Human FceR1a were performed. The affinity constant is 70.0 nM.

FceR1a Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 84662-3-PBS

Clone No.241848D1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

FceRIa, FcERI, Fc-epsilon RI-alpha, FCE1A, Fc epsilon RI alpha

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Product Information

84662-3-PBS targets FceR1a in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Fc fragment of IgE, high affinity I, receptor for; alpha polypeptide
• Calculated Molecular Weight: 30kDa
• Observed Molecular Weight: 55-65 kDa
• GenBank Accession Number: NM_001387280.1
• Gene Symbol: FceR1a
• Gene ID (NCBI): 2205
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P12319
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316). The calculated molecular weight of FceR1a is 30 kDa, the 55- to 70-kDa bands detected by this antibody are probably caused by heterogeneous glycosylation (PMID: 11344350; 12671054).