Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 84702-1-RR
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 84702-1-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HeLa using 84702-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC experiment of HeLa using 84702-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
Dot Blot experiment of peptide using 84702-1-RR
Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
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rat liver tissue, human placenta tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HeLa cells, NIH/3T3 cells, C6 cells
Positive FC (Intra) detected in
HeLa cells
Positive Dot Blot detected in
peptide
This antibody specifically recognize symmetric dimethyl-arginine (SDMA, Rme2s).
Recommended dilution
Application
Dilution
Immunohistochemistry (IHC)
IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC
IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
DOT BLOT
DOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
Product Information
84702-1-RR targets Di-Methyl-Histone H3 (Arg2) in IHC, IF/ICC, FC (Intra), Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat liver using 84702-1-RR
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 84702-1-RR
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84702-1-RR (Di-Methyl-Histone H3 (Arg2) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of NIH/3T3 using 84702-1-RR
Immunofluorescent analysis of (4% PFA) fixed NIH/3T3 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of C6 using 84702-1-RR
Immunofluorescent analysis of (4% PFA) fixed C6 cells using Di-Methyl-Histone H3 (Arg2) antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HeLa using 84702-1-RR
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84702-1-RR, Clone: 241896D7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
FC (INTRA) Figures
FC experiment of HeLa using 84702-1-RR
1x10^6 HeLa cells were intracellularly stained with 0.25 ug Hist1h3a Recombinant Antibody (84702-1-RR, Clone:241896D7) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.
DOT BLOT Figures
Dot Blot experiment of peptide using 84702-1-RR
Dot blot analysis was used to confirm the specificity of 84702-1-RR Di-Methyl-Histone H3 (Arg2) antibody. Peptides were spotted onto NC and probed with antibody at 1 µg/ml. The amount of peptide (ug/mL) spotted is indicated next to each row.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
Di-Methyl-Histone H3 (Arg2) Recombinant antibody
Catalog Number
84702-1-RR
Citations
-
Dilutions
IHC : 1:500-1:2000 IF/ICC : 1:200-1:800 FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension DOT BLOT : 1:10-1:100
Applications
IHC, IF/ICC, FC (Intra), Dot Blot, ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
