WASP Recombinant monoclonal antibody

WASP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 84714-2-RR

Clone No. 242074E4

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

242074E4, Actin nucleation-promoting factor WAS, WAS, Wiskott-Aldrich syndrome protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIF/ICCAffinity

WB analysis using 84714-2-RR
Various lysates were subjected to SDS PAGE followed by western blot with 84714-2-RR (WASP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of Jurkat using 84714-2-RR
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using WASP antibody (84714-2-RR, Clone: 242074E4 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Affinity and Kinetic Characterization of 84714-2-RR
Biolayer interferometry (BLl) kinetic assays of 84714-2-RR against Human WASP were performed. The affinity constant is 1.88 nM.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, TF-1 cells, HL-60 cells, Raji cells
Positive IF/ICC detected in	Jurkat cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84714-2-RR targets WASP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag1444 Product name: Recombinant human WAS protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-163 aa of BC012738 Sequence: MSGGPMGGRPGGRGAPAVQQNIPSTLLQDHENQRLFEMLGRKCLTLATAVVQLYLALPPGAEHWTKEHCGAVCFVKDNPQKSYFIRLYGLQAGRLLWEQELYSQLVYSTPTPFFHTFAGDDCQAGLNFADEDEAQAFRALVQEKIQKRNQRQSGDRRQLPPPP Predict reactive species
Full Name	Wiskott-Aldrich syndrome (eczema-thrombocytopenia)
Calculated Molecular Weight	53 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC012738
Gene Symbol	WASP
Gene ID (NCBI)	7454
RRID	AB_3743675
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P42768
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Wiskott-Aldrich Syndrome protein (WASP) regulates actin cytoskeleton in hematopoietic cells and defects in WASP cause Wiskott-Aldrich Syndrome (WAS), an X-linked immunodeficiency and autoimmunity disorder of childhood.