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  • Western Blot (WB) analysis of mouse brain tissue using GP2 Recombinant antibody (84728-4-RR)

    WB analysis of mouse brain using 84728-4-RR

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 84728-4-RR (GP2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

GP2 Recombinant antibody

GP2 Recombinant Antibody for WB, ELISA
Cat No. 84728-4-RR
Clone No.242300F9

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ZAP75, Pancreatic zymogen granule membrane protein GP-2, 242300F9

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84728-4-RR targets GP2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein Predict reactive species
Full Name glycoprotein 2 (zymogen granule membrane)
Calculated Molecular Weight59 kDa
Observed Molecular Weight70 kDa
GenBank Accession NumberNM_001007240.2
Gene Symbol GP2
Gene ID (NCBI) 2813
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP55259-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Pancreatic secretory granule membrane major glycoprotein (GP2) is the most abundant membrane component of zymogen granules secreted by pancreatic acinar cells (PMID: 8190715). GP2 associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. It is released from the membrane of mature zymogen granules by an enzymatic mechanism (PMID: 2011597). GP2 plays a role in defense against adhesive and invasive commensal bacteria during intestinal inflammation (PMID: 33594081). GP2 is preferentially expressed in acinar cell carcinomas of the pancreas but the glycoprotein can rarely also be expressed in a variety of other tumor entities (PMID: 36137400). The apparent molecular weight of GP2 is 70-100 kDa, which is larger than the calculated molecular weight, due to glycosylation (PMID: 19627615; 30186250).

Protocols

Product Specific Protocols
WB protocol for GP2 antibody 84728-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols