Biotin-conjugated Aggrecan Recombinant monoclonal antibody

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• Biotin-conjugated Aggrecan Recombinant monoclonal antibody (Biotin-84819-5)

Aggrecan Uni-rAb^® Recombinant Antibody for IHC

Cat No. Biotin-84819-5

Clone No.250777C8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC

Synonyms

ACAN, AGC1, AGCAN, Aggrecan 1, Aggrecan core protein 2

• 

  IHC staining of human lung cancer using Biotin-84819-5

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using Biotin-84819-5 (Aggrecan antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

Biotin-84819-5 targets Aggrecan in IHC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2994

  Product name: Recombinant Human ACAN protein (rFc Tag)

  Source: mammalian cells-derived, V37

  Tag: C-rFc

  Domain: 20-675 aa of NM_013227.4

  Sequence: VETSDHDNSLSVSIPQPSPLRVLLGTSLTIPCYFIDPMHPVTTAPSTAPLAPRIKWSRVSKEKEVVLLVATEGRVRVNSAYQDKVSLPNYPAIPSDATLEVQSLRSNDSGVYRCEVMHGIEDSEATLEVVVKGIVFHYRAISTRYTLDFDRAQRACLQNSAIIATPEQLQAAYEDGFHQCDAGWLADQTVRYPIHTPREGCYGDKDEFPGVRTYGIRDTNETYDVYCFAEEMEGEVFYATSPEKFTFQEAANECRRLGARLATTGQLYLAWQAGMDMCSAGWLADRSVRYPISKARPNCGGNLLGVRTVYVHANQTGYPDPSSRYDAICYTGEDFVDIPENFFGVGGEEDITVQTVTWPDMELPLPRNITEGEARGSVILTVKPIFEVSPSPLEPEEPFTFAPEIGATAFAEVENETGEATRPWGFPTPGLGPATAFTSEDLVVQVTAVPGQPHLPGGVVFHYRPGPTRYSLTFEEAQQACLRTGAVIASPEQLQAAYEAGYEQCDAGWLRDQTVRYPIVSPRTPCVGDKDSSPGVRTYGVRPSTETYDVYCFVDRLEGEVFFATRLEQFTFQEALEFCESHNATLATTGQLYAAWSRGLDKCYAGWLADGSLRYPIVTPRPACGGDKPGVRTVYLYPNQTGLPDPLSRHHAFCFR

• Full Name: aggrecan
• Calculated Molecular Weight: 261 kDa
• Observed Molecular Weight: 140-150 kDa
• GenBank Accession Number: NM_013227.4
• Gene Symbol: ACAN
• Gene ID (NCBI): 176
• Conjugate: Biotin
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P16112-1
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Aggrecan (ACAN), also named as CSPCP, AGC1 and MSK16, belongs to the aggrecan/versican proteoglycan family (also including versican, brevican and neurocan). It is a major component of extracellular matrix of cartilaginous tissues. A major function of aggrecan is to resist compression in cartilage. It binds avidly to hyaluronic acid via an N-terminal globular region.