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PDE10A Recombinant monoclonal antibody, PBS Only (Detector) (84869-1-PBS)

Filter: AllWBSandwich ELISA sample testSandwich ELISAFC (Intra)Cytometric bead arrayAffinity

WB analysis using 84869-1-RR (same clone as 84869-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 84869-1-RR (PDE10A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84869-1-PBS in a different storage buffer formulation.
Sandwich ELISA sample test of MP01645-2
The mean PDE10A concentration was determined to be 62.34 ng/mL in HeLa cell extract based on a 1.00 mg/mL extract load, 60.49 ng/mL in Neuro-2a cell extract based on a 1.50 mg/mL extract load and 71.70 ng/mL in SH-SY5Y cell extract based on a 1.00 mg/mL extract load.
Sandwich ELISA standard curve of MP01645-2
Sandwich ELISA standard curve of MP01645-2, Human PDE10A Recombinant Matched Antibody Pair - PBS only. 84869-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag12771. 84869-1-PBS was HRP conjugated as the detection antibody. Range: 0.781-50 ng/mL.
FC experiment of HeLa using 84869-1-RR (same clone as 84869-1-PBS)
1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDE10A Recombinant antibody (84869-1-RR, Clone:242357E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84869-1-PBS in a different storage buffer formulation.
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1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDE10A Recombinant antibody (84869-1-RR, Clone:242357E12) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 84869-1-PBS in a different storage buffer formulation.
Cytometric bead array standard curve of MP01645-2
Cytometric bead array standard curve of MP01645-2, PDE10A Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84869-2-PBS. Detection antibody: 84869-1-PBS. Standard: Ag12771. Range: 0.781-100 ng/mL.
Affinity and Kinetic Characterization of 84869-1-RR
Biolayer interferometry (BLl) kinetic assays of 84869-1-RR against Human PDE10A were performed. The affinity constant is 3.37 nM.

PDE10A Recombinant monoclonal antibody, PBS Only (Detector)

Name: PDE10A Recombinant monoclonal antibody, PBS Only (Detector)
Brand: Proteintech
SKU: 84869-1-PBS

PDE10A Uni-rAb^® Recombinant Antibody for WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Cat No. 84869-1-PBS

Clone No.242357E12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), Cytometric bead array, Sandwich ELISA, Indirect ELISA

Synonyms

242357E12, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A, EC:3.1.4.17, HSPDE10A, phosphodiesterase 10A

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

84869-1-PBS targets PDE10A as part of a matched antibody pair:

MP01645-2: 84869-2-PBS capture and 84869-1-PBS detection (validated in Cytometric bead array, Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag12771 Product name: Recombinant human PDE10A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 434-779 aa of BC104860 Sequence: KLSYHSICTSEEWQGLMQFTLPVRLCKEIELFHFDIGPFENMWPGIFVYMVHRSCGTSCFELEKLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNHTLFTDLERKGLLIACLCHDLDHRGFSNSYLQKFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLEEMYQTGSLNLNNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDRDKKDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLSQWEKVIRGEETATWISSPSVAQKAAASED Predict reactive species
Full Name	phosphodiesterase 10A
Calculated Molecular Weight	779 aa, 88 kDa
Observed Molecular Weight	80 kDa
GenBank Accession Number	BC104860
Gene Symbol	PDE10A
Gene ID (NCBI)	10846
RRID	AB_3743794
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q9Y233
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).