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  • Western Blot (WB) analysis of various lysates using Phospho-EGFR (Tyr1197) Recombinant monoclonal anti (84906-1-RR)

    WB analysis using 84906-1-RR

    Non-treated A431 cells and EGF (HZ-1326) treated A431 cells were subjected to SDS PAGE followed by western blot with 84906-1-RR (Phospho-EGFR (Tyr1197) antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with Alpha Tubulin (66031-1-Ig) antibody as a loading control.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using Phospho-EGFR (Tyr1197) Recombinant monoclonal anti (84906-1-RR)

    IF Staining of A431 using 84906-1-RR

    Immunofluorescent analysis of (4% PFA) fixed EGF treated A431 cells using Phospho-EGFR (Tyr1197) antibody (84906-1-RR, Clone: 241826G10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of A431 cells using Phospho-EGFR (Tyr1197) Recombinant antibody (84906-1-RR)

    FC experiment of A431 using 84906-1-RR

    1x10^6 A431 cells untreated (dashed lines) or treated with EGF which intracellularly stained with 0.25 ug Phospho-EGFR (Tyr1197) Recombinant Antibody (84906-1-RR, Clone:241826G10) and PE-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with 90% MeOH.

Phospho-EGFR (Tyr1197) Recombinant monoclonal antibody

Phospho-EGFR (Tyr1197) Uni-rAb® Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84906-1-RR
Clone No.241826G10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

EGF Receptor (Tyr1173), EGFR (Tyr1197), p-EGF Receptor, p-EGF Receptor (Tyr1173), p-EGFR

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inEGF treated A431 cells
Positive FC (Intra) detected inEGF treated A431 cells

This antibody, also known as EGFR Tyr1173, is the site where the signal peptide is removed.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84906-1-RR targets Phospho-EGFR (Tyr1197) in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

Predict reactive species
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Calculated Molecular Weight 1210 aa, 134 kDa
Observed Molecular Weight175 kDa
GenBank Accession NumberBC094761
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDP00533
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). The EGFR autophosphorylation sites Tyr-992 and Tyr-1173 (also known as Tyr 1197) have been shown to play a critical role in the activation of the MAPK cascade following EGF stimulation. (PMID: 15708852) Some scientists call EGFR Tyr1197 as EGFR Tyr1173, the site where the signaling peptide is removed.

Protocols

Product Specific Protocols
WB protocol for Phospho-EGFR (Tyr1197) antibody 84906-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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