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  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TCOF1 Recombinant antibody (84914-5-RR)

    IHC staining of human colon cancer using 84914-5-RR

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84914-5-RR (TCOF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TCOF1 Recombinant antibody (84914-5-RR)

    IF Staining of MCF-7 using 84914-5-RR

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-5-RR, Clone: 242201F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 84914-5-RR

    Affinity and Kinetic Characterization of 84914-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84914-5-RR against Human TCOF1 were performed. The affinity constant is 8.67 nM.

TCOF1 Recombinant antibody

TCOF1 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, ELISA

Cat No. 84914-5-RR
Clone No.242201F12

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

242201F12, MFD1, treacle

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84914-5-RR targets TCOF1 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen TCOF1 fusion protein Ag1447 Predict reactive species
Full Name Treacher Collins-Franceschetti syndrome 1
Calculated Molecular Weight 97 kDa, 144 kDa, 152 kDa
GenBank Accession NumberBC014559
Gene Symbol TCOF1
Gene ID (NCBI) 6949
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13428
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)

Protocols

Product Specific Protocols
IHC protocol for TCOF1 antibody 84914-5-RRDownload protocol
IF protocol for TCOF1 antibody 84914-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols