TCOF1 Recombinant monoclonal antibody, PBS Only

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  IHC staining of human colon cancer using 84914-5-RR (same clone as 84914-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84914-5-RR (TCOF1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84914-5-PBS in a different storage buffer formulation.

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  IF Staining of MCF-7 using 84914-5-RR (same clone as 84914-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (84914-5-RR, Clone: 242201F12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84914-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84914-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84914-5-RR against Human TCOF1 were performed. The affinity constant is 8.67 nM.

TCOF1 Uni-rAb^® Recombinant Antibody for IHC, IF/ICC, Indirect ELISA

Cat No. 84914-5-PBS

Clone No.242201F12

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

242201F12, MFD1, treacle

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Product Information

84914-5-PBS targets TCOF1 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag1447

  Product name: Recombinant human TCOF1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC014559

  Sequence: MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSE

• Full Name: Treacher Collins-Franceschetti syndrome 1
• Calculated Molecular Weight: 97 kDa, 144 kDa, 152 kDa
• GenBank Accession Number: BC014559
• Gene Symbol: TCOF1
• Gene ID (NCBI): 6949
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q13428
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)