CoraLite® Plus 488-conjugated CSF1R/CD115 Recombinant monoclonal antibody

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  IF Staining of RAW 264.7 using CL488-84919

  Immunofluorescent analysis of (-20°C Ethanol) fixed RAW 264.7 cells using CoraLite® Plus 488 CSF1R/CD115 antibody (CL488-84919, Clone: 242213B1 ) at dilution of 1:200.

CSF1R/CD115 Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-84919

Clone No.242213B1

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC

Synonyms

Csf1r, CD115, CSF 1R, CSF-1 receptor, Csfmr

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Tested Applications

• Positive IF/ICC detected in: RAW 264.7 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-84919 targets CSF1R/CD115 in IF/ICC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg1816

  Product name: Recombinant Mouse CSF1R protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 20-511 aa of NM_001037859.2

  Sequence: APVIEPSGPELVVEPGETVTLRCVSNGSVEWDGPISPYWTLDPESPGSTLTTRNATFKNTGTYRCTELEDPMAGSTTIHLYVKDPAHSWNLLAQEVTVVEGQEAVLPCLITDPALKDSVSLMREGGRQVLRKTVYFFSPWRGFIIRKAKVLDSNTYVCKTMVNGRESTSTGIWLKVNRVHPEPPQIKLEPSKLVRIRGEAAQIVCSATNAEVGFNVILKRGDTKLEIPLNSDFQDNYYKKVRALSLNAVDFQDAGIYSCVASNDVGTRTATMNFQVVESAYLNLTSEQSLLQEVSVGDSLILTVHADAYPSIQHYNWTYLGPFFEDQRKLEFITQRAIYRYTFKLFLNRVKASEAGQYFLMAQNKAGWNNLTFELTLRYPPEVSVTWMPVNGSDVLFCDVSGYPQPSVTWMECRGHTDRCDEAQALQVWNDTHPEVLSQKPFDKVIIQSQLPIGTLKHNMTYFCKTHNSVGNSSQYFRAVSLGQSKQLPDES

• Full Name: colony stimulating factor 1 receptor
• Calculated Molecular Weight: 109 kDa
• Observed Molecular Weight: 110-170 kDa
• GenBank Accession Number: NM_001037859.2
• Gene Symbol: CSF1R/CD115
• Gene ID (NCBI): 12978
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P09581
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

CSF1R (colony-stimulating factor 1 receptor) belongs to the type III protein tyrosine kinase receptor family. CSF1R-mediated signaling is crucial for the differentiation and survival of the mononuclear phagocyte system and macrophages in particular (PMID: 28716061).