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  • Western Blot (WB) analysis of various lysates using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    WB analysis using 85018-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85018-4-RR (SMARCA2-Specific antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SMARCA2-Specific Recombinant antibody (85018-4-RR)

    IF Staining of HeLa using 85018-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SMARCA2-Specific antibody (85018-4-RR, Clone: 242511E1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85018-4-RR

    Affinity and Kinetic Characterization of 85018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85018-4-RR against Human SMARCA2-Specific were performed. The affinity constant is 3.86 nM.

SMARCA2-Specific Recombinant antibody

SMARCA2-Specific Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85018-4-RR
Clone No.242511E1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

SMARCA2, BRM, BRG1-associated factor 190B, BAF190B, BAF190

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, U-251 cells, MCF-7 cells, K-562 cells, NIH/3T3 cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85018-4-RR targets SMARCA2-Specific in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SMARCA2-Specific fusion protein Ag25157 Predict reactive species
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
Calculated Molecular Weight 181 kDa
Observed Molecular Weight210 kDa
GenBank Accession NumberNM_003070
Gene Symbol SMARCA2
Gene ID (NCBI) 6595
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP51531
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.

Protocols

Product Specific Protocols
WB protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
IF protocol for SMARCA2-Specific antibody 85018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols