CoraLite® Plus 488-conjugated SMARCA2-Specific Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-85018-4

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 SMARCA2-Specific antibody (CL488-85018-4, Clone: 242511E1 ) at dilution of 1:200, CL594-phalloidin (red).

SMARCA2-Specific Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-85018-4

Clone No.242511E1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

SMARCA2, ATP-dependent helicase SMARCA2, BAF190, BAF190B, BRG1-associated factor 190B

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-85018-4 targets SMARCA2-Specific in IF/ICC applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag25157

  Product name: Recombinant human SMARCA2-Specific protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-272 aa of NM_003070

  Sequence: MSTPTDPGAMPHPGPSPGPGPSPGPILGPSPGPGPSPGSVHSMMGPSPGPPSVSHPMPTMGSTDFPQEGMHQMHKPIDGIHDKGIVEDIHCGSMKGTGMRPPHPGMGPPQSPMDQHSQGYMSPHPSPLGAPEHVSSPMSGGGPTPPQMPPSQPGALIPGDPQAMSQPNRGPSPFSPVQLHQLRAQILAYKMLARGQPLPETLQLAVQGKRTLPGLQQQQQQQQQQQQQQQQQQQQQQQPQQQPPQPQTQQQQQPALVNYNRPSGPGPELSGP

• Full Name: SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 2
• Calculated Molecular Weight: 181 kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: NM_003070
• Gene Symbol: SMARCA2
• Gene ID (NCBI): 6595
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P51531
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

SMARCA2 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. SMARCA2 is involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.