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  • Western Blot (WB) analysis of various lysates using PDLIM1/CLP36 Recombinant antibody (85027-5-RR)

    WB analysis using 85027-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85027-5-RR (PDLIM1,CLP36 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDLIM1/CLP36 Recombinant antibody (85027-5-RR)

    IF Staining of HeLa using 85027-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDLIM1/CLP36 antibody (85027-5-RR, Clone: 242585C7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of HepG2 cells using PDLIM1/CLP36 Recombinant antibody (85027-5-RR)

    FC experiment of HepG2 using 85027-5-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PDLIM1/CLP36 Recombinant antibody (85027-5-RR, Clone:242585C7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 85027-5-RR

    Affinity and Kinetic Characterization of 85027-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85027-5-RR against Human PDLIM1/CLP36 were performed. The affinity constant is 6.23 nM.

PDLIM1/CLP36 Recombinant antibody

PDLIM1/CLP36 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85027-5-RR
Clone No.242585C7

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

PDLIM1, 242585C7, CLIM1, CLP36, CLP-36

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inC2C12 cells, Caco-2 cells, HEK-293T cells, Saos-2 cells, rat lung tissue, human heart tissue
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85027-5-RR targets PDLIM1/CLP36 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PDLIM1/CLP36 fusion protein Ag2274 Predict reactive species
Full Name PDZ and LIM domain 1
Calculated Molecular Weight 329 aa, 36 kDa
Observed Molecular Weight 36 kDa
GenBank Accession NumberBC000915
Gene Symbol PDLIM1/CLP36
Gene ID (NCBI) 9124
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO00151
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDLIM1, also named as CLIM1, CLP-36, CLP36, hCLIM1 and Elfin, is a member of the actinin-associated LIM protein (ALP)/enigma protein family, plays a role in neurite outgrowth in the peripheral nervous system. It is a cytoskeletal protein that may act as an adapter that brings other proteins (like kinases) to the cytoskeleton. PDLIM1 and PALLD formed a complex in the dorsal root ganglion (DRG) and PDLIM1 and PALLD were colocalized in the neurites and cell bodies of primary DRG neurons. PDLIM1 may interact with PALLD to influence neurite outgrowth during sciatic nerve regeneration. (PMID: 20381583)

Protocols

Product Specific Protocols
WB protocol for PDLIM1/CLP36 antibody 85027-5-RRDownload protocol
IF protocol for PDLIM1/CLP36 antibody 85027-5-RRDownload protocol
FC protocol for PDLIM1/CLP36 antibody 85027-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols