Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, HEK-293 cells, U-87 MG cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85032-2-RR targets RFK in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag8591 Product name: Recombinant human RFK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-162 aa of BC007069 Sequence: MPRADCIMRHLPYFCRGQVVRGFGRGSKQLGIPTANFPEQVVDNLPADISTGIYYGWASVGSGDVHKMVVSIGWNPYYKNTKKSMETHIMHTFKEDFYGEILNVAIVGYLRPEKNFDSLESLISAIQGDIEEAKKRLELPEHLKIKEDNFFQVSKSKIMNGH Predict reactive species |
| Full Name | riboflavin kinase |
| Calculated Molecular Weight | 162 aa, 18 kDa |
| GenBank Accession Number | BC007069 |
| Gene Symbol | RFK |
| Gene ID (NCBI) | 55312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q969G6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Riboflavin kinase (RFK) is an essential enzyme that catalyzes the phosphorylation of riboflavin (vitamin B2) to form flavin mononucleotide (FMN), an obligatory step in vitamin B2 utilization and flavin cofactor synthesis(PMID:12623014). RFK is rate-limiting in the synthesis of FAD, an essential prosthetic group of NADPH oxidase.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RFK antibody 85032-2-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
