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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PML Recombinant antibody (85048-5-RR)

    IF Staining of HeLa using 85048-5-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PML antibody (85048-5-RR, Clone: 242564C10 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using PML Recombinant antibody (85048-5-RR)

    FC experiment of A431 using 85048-5-RR

    1x10^6 A431 cells were intracellularly stained with 0.25 ug PML Recombinant antibody (85048-5-RR, Clone:242564C10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

  • Affinity and Kinetic Characterization of 85048-5-RR

    Affinity and Kinetic Characterization of 85048-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85048-5-RR against Human PML were performed. The affinity constant is below 1 pM.

PML Recombinant antibody

PML Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), ELISA
Cat No. 85048-5-RR
Clone No.242564C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), ELISA

Synonyms

242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85048-5-RR targets PML in IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PML fusion protein Ag15377 Predict reactive species
Full Name promyelocytic leukemia
Calculated Molecular Weight 882 aa, 98 kDa
GenBank Accession NumberBC020994
Gene Symbol PML
Gene ID (NCBI) 5371
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP29590
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.

Protocols

Product Specific Protocols
IF protocol for PML antibody 85048-5-RRDownload protocol
FC protocol for PML antibody 85048-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols