CoraLite®594-conjugated PML Recombinant monoclonal antibody

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  IF Staining of HeLa using CL594-85048-5

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite®594 PML antibody (CL594-85048-5, Clone: 242564C10 ) at dilution of 1:200, CL488-phalloidin (green).

PML Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL594-85048-5

Clone No.242564C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC

Synonyms

242564C10, E3 SUMO-protein ligase PML, EC:2.3.2.-, Protein PML, RING-type E3 SUMO transferase PML

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-85048-5 targets PML in IF/ICC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag15377

  Product name: Recombinant human PML protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 73-418 aa of BC020994

  Sequence: LHTLCSGCLEASGMQCPICQAPWPLGADTPALDNVFFESLQRRLSVYRQIVDAQAVCTRCKESADFWCFECEQLLCAKCFEAHQWFLKHEARPLAELRNQSVREFLDGTRKTNNIFCSNPNHRTPTLTSIYCRGCSKPLCCSCALLDSSHSDLKCDISAEIQQRQEELDAMTQALQEQDSAFGAVHAQMHAAVGQLGRARAETEELIRERVRQVVAHVRAQERELLEAVDARYQRDYEEMASRLGRLDAVLQRIRTGSALVQRMKCYASDQEVLDMHGFLRQALCRLRQEEPQSLQAAVRTDGFDEFKVRLQDLSSCITQGKDAAVSKKASPEAASTPRDPIDVDL

• Full Name: promyelocytic leukemia
• Calculated Molecular Weight: 882 aa, 98 kDa
• GenBank Accession Number: BC020994
• Gene Symbol: PML
• Gene ID (NCBI): 5371
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P29590
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PML, also named as MYL, RNF71 and TRIM19, is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. It functions as tumor suppressor. PML is required for normal, caspase-dependent apoptosis in response to DNA damage, FAS, TNF, or interferons. It plays a role in transcription regulation, DNA damage response, DNA repair and chromatin organization. PML plays a role in processes regulated by retinoic acid, regulation of cell division, terminal differentiation of myeloid precursor cells and differentiation of neural progenitor cells. It regulates PTEN compartmentalization through the inhibition of USP7-mediated deubiquitinylation.