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  • Western Blot (WB) analysis of various lysates using STRN Recombinant antibody (85058-4-RR)

    WB analysis using 85058-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85058-4-RR (STRN antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IHC staining of mouse brain using 85058-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85058-4-RR (STRN antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IHC staining of mouse brain using 85058-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85058-4-RR (STRN antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IF Staining of mouse brain using 85058-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STRN antibody (85058-4-RR, Clone: 242532D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using STRN Recombinant antibody (85058-4-RR)

    IF Staining of mouse brain using 85058-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using STRN antibody (85058-4-RR, Clone: 242532D8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 85058-4-RR

    Affinity and Kinetic Characterization of 85058-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85058-4-RR against Human STRN were performed. The affinity constant is 0.433 nM.

STRN Recombinant antibody

STRN Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 85058-4-RR
Clone No.242532D8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Striatin, SG2NA, 242532D8

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inA549 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85058-4-RR targets STRN in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen STRN fusion protein Ag15984 Predict reactive species
Full Name striatin, calmodulin binding protein
Calculated Molecular Weight 780 aa, 86 kDa
Observed Molecular Weight 110 kDa
GenBank Accession NumberBC106879
Gene Symbol STRN
Gene ID (NCBI) 6801
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

STRN (Striatin), a 780-amino acid protein, was identified and cloned more than a decade ago. In adult mammals, striatin is mainly expressed in neurons of both the central and peripheral nervous systems with very high levels of expression in the striatum, hence its name. STRN-ALK fusion may be a potential therapeutic target in the aggressive forms of thyroid cancer (PMID: 24475247).

Protocols

Product Specific Protocols
WB protocol for STRN antibody 85058-4-RRDownload protocol
IHC protocol for STRN antibody 85058-4-RRDownload protocol
IF protocol for STRN antibody 85058-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols