• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of MCF-7 cells using Progesterone Receptor Recombinant antibody (85112-4-RR)

    WB analysis of MCF-7 using 85112-4-RR

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 85112-4-RR (PR antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Progesterone Receptor Recombinant antibody (85112-4-RR)

    WB analysis using 85112-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85112-4-RR (Progesterone Receptor antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IHC staining of human cervical cancer using 85112-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IHC staining of human cervical cancer using 85112-4-RR

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 85112-4-RR (PR antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of T-47D cells using Progesterone Receptor Recombinant antibody (85112-4-RR)

    IF Staining of T-47D using 85112-4-RR

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells, MCF-7 cells, MDA-MB-231 cells using PR antibody (85112-4-RR, Clone: 242686G1 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 85112-4-RR

    Affinity and Kinetic Characterization of 85112-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85112-4-RR against Human Progesterone Receptor were performed. The affinity constant is 0.587 nM.

Progesterone Receptor Recombinant antibody

Progesterone Receptor Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 85112-4-RR
Clone No.242686G1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PGR, NR3C3, PR, PRGR

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inMCF-7 cells, T-47D cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85112-4-RR targets Progesterone Receptor in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Progesterone Receptor fusion protein Ag22986 Predict reactive species
Full Name progesterone receptor
Calculated Molecular Weight 933 aa, 99 kDa
Observed Molecular Weight 118 kDa
GenBank Accession NumberNM_000926
Gene Symbol Progesterone Receptor
Gene ID (NCBI) 5241
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP06401
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence.

Protocols

Product Specific Protocols
WB protocol for Progesterone Receptor antibody 85112-4-RRDownload protocol
IHC protocol for Progesterone Receptor antibody 85112-4-RRDownload protocol
IF protocol for Progesterone Receptor antibody 85112-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols