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  • Western Blot (WB) analysis of various lysates using INPP4B Recombinant antibody (85151-4-RR)

    WB analysis using 85151-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85151-4-RR (INPP4B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using INPP4B Recombinant antibody (85151-4-RR)

    IF Staining of MCF-7 using 85151-4-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using INPP4B antibody (85151-4-RR, Clone: 242837H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Flow cytometry (FC) experiment of K562 using INPP4B Recombinant antibody (85151-4-RR)

    FC experiment of K562 using 85151-4-RR

    1x10^6 K562 were intracellularly stained with 0.25 ug INPP4B Recombinant antibody (85151-4-RR, Clone:242837H2) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • Affinity and Kinetic Characterization of 85151-4-RR

    Affinity and Kinetic Characterization of 85151-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85151-4-RR against Human INPP4B were performed. The affinity constant is 0.563 nM.

INPP4B Recombinant monoclonal antibody

INPP4B Uni-rAb® Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA
Cat No. 85151-4-RR
Clone No.242837H2

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, FC (Intra), ELISA

Synonyms

242837H2, EC:3.1.3.66, Inositol polyphosphate 4-phosphatase type II, Type II inositol 3,4-bisphosphate 4-phosphatase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inLNCaP cells, A549 cells, HeLa cells, MCF-7 cells
Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inK562

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85151-4-RR targets INPP4B in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag8676

Product name: Recombinant human INPP4B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-53 aa of BC005273

Sequence: MEIKEEGASEEGQHFLPTAQANDPGDCQFTSIQKTPNEPQLEFILDLNQELRD

Predict reactive species
Full Name inositol polyphosphate-4-phosphatase, type II, 105kDa
Calculated Molecular Weight 53 aa, 6 kDa
Observed Molecular Weight 100-110 kDa
GenBank Accession NumberBC005273
Gene Symbol INPP4B
Gene ID (NCBI) 8821
Conjugate Unconjugated
FormLiquid
Purification MethodProtein A purification
UNIPROT IDO15327
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

INPP4B (Inositol polyphosphate 4-phosphatase type II) can catalyze the hydrolysis of the 4-position phosphate of phosphatidylinositol 3,4-bisphosphate, inositol 1,3,4-trisphosphate and inositol 3,4-trisphosphate (PMID: 24070612; 24591580). It also Plays a role in the late stages of macropinocytosis by dephosphorylating phosphatidylinositol 3,4-bisphosphate in membrane ruffles (PMID: 24591580).

Protocols

Product Specific Protocols
FC protocol for INPP4B antibody 85151-4-RRDownload protocol
IF protocol for INPP4B antibody 85151-4-RRDownload protocol
WB protocol for INPP4B antibody 85151-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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