HeLa untreated and IFN gamma treated cells lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
HeLa untreated and IFN gamma treated cells lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 85254-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
IRF1, also named as IFN regulatory factor 1, is a 325 amino acid protein, which belongs to the IRF family. IRF1 as a transcriptional regulator which displays a remarkable functional diversity in the regulation of cellular responses. The calculated molecular weight of IRF1 is 37 kDa, but the modified IRF1 protein is about 45-50 kDa.
HeLa untreated and IFN gamma treated cells lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 85254-3-RR
Various lysates were subjected to SDS PAGE followed by western blot with 85254-3-RR (IRF1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
IRF1 Recombinant antibody
Catalog Number
85254-3-RR
Citations
-
Dilutions
WB : 1:2000-1:10000
Applications
WB, ELISA
Reactivity
human
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
