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  • Western Blot (WB) analysis of HepG2 cells using Phospho-SMAD1 (Ser187) Recombinant antibody (85388-4-RR)

    WB analysis of HepG2 using 85388-4-RR

    Non-treated HepG2 cells and BMP2 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 85388-4-RR (Phospho-SMAD1 (Ser187) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control.

Phospho-SMAD1 (Ser187) Recombinant antibody

Phospho-SMAD1 (Ser187) Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85388-4-RR
Clone No.250292C10

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Phospho SMAD1 (Ser187), Phospho SMAD1(Ser187), Phospho-SMAD1, Phospho-SMAD1(Ser187), Phospho-SMAD1-Ser187

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inBMP2 treated HepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85388-4-RR targets Phospho-SMAD1 (Ser187) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide Predict reactive species
Full Name SMAD family member 1
Calculated Molecular Weight 52 kDa
GenBank Accession NumberBC001878
Gene Symbol SMAD1
Gene ID (NCBI) 4086
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15797
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS).

Protocols

Product Specific Protocols
WB protocol for Phospho-SMAD1 (Ser187) antibody 85388-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols