Phospho-SMAD1 (Ser187) Recombinant antibody, PBS Only

• 

  WB analysis of HepG2 using 85388-4-RR (same clone as 85388-4-PBS)

  Non-treated HepG2 cells and BMP2 treated HepG2 cells were subjected to SDS PAGE followed by western blot with 85388-4-RR (Phospho-SMAD1 (Ser187) antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) antibody as a loading control. This data was developed using the same antibody clone with 85388-4-PBS in a different storage buffer formulation.

Phospho-SMAD1 (Ser187) Uni-rAb^TM Recombinant Antibody for WB, Indirect ELISA

Cat No. 85388-4-PBS

Clone No.250292C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

Phospho SMAD1 (Ser187), Phospho SMAD1(Ser187), Phospho-SMAD1, Phospho-SMAD1(Ser187), Phospho-SMAD1-Ser187

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Product Information

85388-4-PBS targets Phospho-SMAD1 (Ser187) in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: SMAD family member 1
• Calculated Molecular Weight: 52 kDa
• GenBank Accession Number: BC001878
• Gene Symbol: SMAD1
• Gene ID (NCBI): 4086
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q15797
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Transforming growth factor-β (TGF-β) superfamily is recognized as one of the largest families of secreted multifunctional peptides exerting different biological effects on a large variety of cell types, such as regulation of hormone secretion, stimulation of extracellular matrix formation, the inhibition of proliferation of many cell types, cell survival, bone formation, and chemotaxis for inflammatory cells. One of the most important proteins that modulate TGF-β ligand activity is the SMAD family proteins. SMAD1 is one of the receptor-activated Smads. It's also a signal transducers of BMP signaling and binds to several proteins involved in ubiquitin-proteasome system (UPS).