Tested Applications
| Positive WB detected in | MCF-7 cells, HeLa cells, HepG2 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
85424-1-RR targets TXNRD2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag8367 Product name: Recombinant human TXNRD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 137-485 aa of BC007489 Sequence: EILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG Predict reactive species |
| Full Name | thioredoxin reductase 2 |
| Calculated Molecular Weight | 524 aa, 56 kDa |
| Observed Molecular Weight | 54 kDa |
| GenBank Accession Number | BC007489 |
| Gene Symbol | TXNRD2 |
| Gene ID (NCBI) | 10587 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9NNW7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TXNRD2 antibody 85424-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
