TXNRD2 Recombinant monoclonal antibody, PBS Only

• 

  WB analysis using 85424-1-RR (same clone as 85424-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85424-1-RR (TXNRD2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85424-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85424-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85424-1-RR against Human TXNRD2 were performed. The affinity constant is below 1 pM.

TXNRD2 Uni-rAb^® Recombinant Antibody for WB, Indirect ELISA

Cat No. 85424-1-PBS

Clone No.242912F11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

TRXR2, 242912F11, EC:1.8.1.9, KIAA1652, Selenoprotein Z

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Product Information

85424-1-PBS targets TXNRD2 in WB, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag8367

  Product name: Recombinant human TXNRD2 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 137-485 aa of BC007489

  Sequence: EILLSADHIIIATGGRPRYPTHIEGALEYGITSDDIFWLKESPGKTLVVGASYVALECAGFLTGIGLDTTIMMRSIPLRGFDQQMSSMVIEHMASHGTRFLRGCAPSRVRRLPDGQLQVTWEDSTTGKEDTGTFDTVLWAIGRVPDTRSLNLEKAGVDTSPDTQKILVDSREATSVPHIYAIGDVVEGRPELTPIAIMAGRLLVQRLFGGSSDLMDYDNVPTTVFTPLEYGCVGLSEEEAVARHGQEHVEVYHAHYKPLEFTVAGRDASQCYVKMVCLREPPQLVLGLHFLGPNAGEVTQGFALGIKCGASYAQVMRTVGIHPTCSEEVVKLRISKRSGLDPTVTGCUG

• Full Name: thioredoxin reductase 2
• Calculated Molecular Weight: 524 aa, 56 kDa
• Observed Molecular Weight: 54 kDa
• GenBank Accession Number: BC007489
• Gene Symbol: TXNRD2
• Gene ID (NCBI): 10587
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NNW7
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Human mitochondrial thioredoxin reductase (TXNRD2) is a selenocysteine-containing enzyme essential for mitochondrial oxygen radical scavenging. Three mammalian thioredoxin reductases exist; a cytosolic (TXNRD1), a mitochondrial (TXNRD2), and a testis-specific thioredoxin reductase (TXNRD3). (PMID: 21247928, PMID: 26199228)