• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
Filter:
  • Western Blot (WB) analysis of various lysates using SRPK2 Recombinant antibody (85613-5-RR)

    WB analysis using 85613-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85613-5-RR (SRPK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 85613-5-RR

    Affinity and Kinetic Characterization of 85613-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85613-5-RR against Human SRPK2 were performed. The affinity constant is 4.16 nM.

SRPK2 Recombinant antibody

SRPK2 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85613-5-RR
Clone No.242935B1

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

EC:2.7.11.1, Serine/arginine-rich protein-specific kinase 2, SFRS protein kinase 2, SFRSK2, SR protein specific kinase 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Please visit your regions distributor:

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, Jurkat cells, K-562 cells, MCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85613-5-RR targets SRPK2 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SRPK2 fusion protein Ag17885 Predict reactive species
Full Name SFRS protein kinase 2
Calculated Molecular Weight 688 aa, 78 kDa
Observed Molecular Weight 105-110 kDa
GenBank Accession NumberBC035214
Gene Symbol SRPK2
Gene ID (NCBI) 6733
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP78362
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRPK2, also named as serine/arginine protein kinase 2 and SFRSK2, belongs to SR protein-specific kinase family which phosphorylate the serine/arginine domain containing proteins in the nuclear speckles to mediate pre-mRNA splicing (PMID: 22854045). In addition to its major role as kinase for the phosphorylation of SR proteins, SRPK2 is involved in cell cycle regulation and cell apoptosis (PMID: 19592491). SRPK2 promotes the proliferation and migration of the colon cancer cells. It has been reported to be regulated in several cancer types (PMID: 27041240). It has 2 isoforms with the calculated molecular mass of 77-79 kDa, and the apparent molecular mass of 105-115 kDa due to posttranslational modification (PMID: 12134018, 16122776).

Protocols

Product Specific Protocols
WB protocol for SRPK2 antibody 85613-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols