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ST3GAL2 Recombinant monoclonal antibody, PBS Only (Detector) (85634-2-PBS)

ST3GAL2 Recombinant monoclonal antibody, PBS Only (Detector)

Name: ST3GAL2 Recombinant monoclonal antibody, PBS Only (Detector), 100ug
Brand: Proteintech
SKU: 85634-2-PBS-100UG

ST3GAL2 Uni-rAb^® Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Cat No. 85634-2-PBS

Clone No.253479G5

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Synonyms

Alpha 2,3-ST 2, Beta-galactoside alpha-2,3-sialyltransferase 2, CMP-N-acetylneuraminate-beta-galactosamide-alpha-2,3-sialyltransferase 2, EC:2.4.3.2, EC:2.4.3.4

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

SKU:

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Freight/Packing: -

Quantity

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Filter: AllWBIHCCytometric bead array sample testCytometric bead array

WB analysis using 85634-2-RR (same clone as 85634-2-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 85634-2-RR (ST3GAL2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85634-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 85634-2-RR (same clone as 85634-2-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85634-2-RR (ST3GAL2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85634-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 85634-2-RR (same clone as 85634-2-PBS)
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85634-2-RR (ST3GAL2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85634-2-PBS in a different storage buffer formulation.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array sample test of MP03241-1
The mean ST3GAL2 concentration was determined to be 15.8 ng/mL in HT-29 cell extract based on a 1.2 mg/mL extract load, 0.5 ng/mL in A431 cell extract based on a 1.2 mg/mL extract load.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
Cytometric bead array standard curve of MP03241-1
Cytometric bead array standard curve of MP03241-1, ST3GAL2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85634-3-PBS. Detection antibody: 85634-2-PBS. Standard: Ag4528. Range: 1.562-200 ng/mL.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

85634-2-PBS targets ST3GAL2 as part of a matched antibody pair:

MP03241-1: 85634-3-PBS capture and 85634-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Recombinant
Type	Antibody
Immunogen	CatNo: Ag4528 Product name: Recombinant human ST3GAL2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 27-210 aa of BC036777 Sequence: SHHSMATLPYLDSGALDGTHRVKLVPGYAGLQRLSKERLSGKSCACRRCMGDAGASDWFDSHFDGNISPVWTRENMDLPPDVQRWWMMLQPQFKSHNTNEVLEKLFQIVPGENPYRFRDPHQCRRCAVVGNSGNLRGSGYGQDVDGHNFIMRMNQAPTVGFEQDVGSRTTHHFMYPESAKNLPA Predict reactive species
Full Name	ST3 beta-galactoside alpha-2,3-sialyltransferase 2
Calculated Molecular Weight	350 aa, 40 kDa
Observed Molecular Weight	40-43 kDa
GenBank Accession Number	BC036777
Gene Symbol	ST3GAL2
Gene ID (NCBI)	6483
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	Q16842
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Beta-galactoside alpha-2,3-sialyltransferase 2 (ST3GAL2) is a sialyltransferase (ST) that transfers sialic acid from the activated donor CMP-sialic acid to the glycan terminus of a glycolipid or glycoprotein. ST3GAL2 contains two putative N-glycosylation sites (Asn92 and Asn211), which are mainly responsible for GD1a and GT1b ganglioside biosynthesis in the brain. ST3GAL2 belongs to the sialyltransferase family, which mediates the terminal modification of glycoproteins and glycolipids. ST3GAL2 has been found to play a role in obesity, aging, and malignant diseases (PMID: 34319453).