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  • Western Blot (WB) analysis of various lysates using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    WB analysis using 85664-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    IF Staining of HeLa using 85664-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using SII-TFIIS,TCEA1 Recombinant antibody (85664-4-RR)

    IF Staining of A431 using 85664-4-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

SII-TFIIS,TCEA1 Recombinant antibody

SII-TFIIS,TCEA1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 85664-4-RR
Clone No.243155H8

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

TCEA1, GTF2S, SII, SII TFIIS, TCEA

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

- +

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Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, Jurkat cells, SGC-7901 cells, K-562 cells, Sp2/0 cells
Positive IF/ICC detected inA431 cells, HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85664-4-RR targets SII-TFIIS,TCEA1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen SII-TFIIS,TCEA1 fusion protein Ag12199 Predict reactive species
Full Name transcription elongation factor A (SII), 1
Calculated Molecular Weight 301 aa, 34 kDa
Observed Molecular Weight40 kDa
GenBank Accession NumberBC072460
Gene Symbol TCEA1
Gene ID (NCBI) 6917
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP23193
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.

Protocols

Product Specific Protocols
WB protocol for SII-TFIIS,TCEA1 antibody 85664-4-RRDownload protocol
IF protocol for SII-TFIIS,TCEA1 antibody 85664-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols