SII-TFIIS,TCEA1 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 85664-4-RR (same clone as 85664-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85664-4-RR (SII-TFIIS,TCEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 85664-4-RR (same clone as 85664-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 85664-4-RR (same clone as 85664-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using SII-TFIIS,TCEA1 antibody (85664-4-RR, Clone: 243155H8 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85664-4-PBS in a different storage buffer formulation.

SII-TFIIS,TCEA1 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 85664-4-PBS

Clone No.243155H8

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

TCEA1, GTF2S, SII, SII TFIIS, TCEA

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Product Information

85664-4-PBS targets SII-TFIIS,TCEA1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12199

  Product name: Recombinant human SII-TFIIS,TCEA1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-301 aa of BC072460

  Sequence: MEDEVVRFAKKMDKMVQKKNAAGALDLLKELKNIPMTLELLQSTRIGMSVNAIRKQSTDEEVTSLAKSLIKSWKKLLDGPSTEKDLDEKKKEPAITSQNSPEAREESTSSGNVSNRKDETNARDTYVSSFPRAPSTSDSVRLKCREMLAAALRTGDDYIAIGADEEELGSQIEEAIYQEIRNTDMKYKNRVRSRISNLKDAKNPNLRKNVLCGNIPPDLFARMTAEEMASDELKEMRKNLTKEAIREHQMAKTGGTQTDLFTCGKCKKKNCTYTQVQTRSADEPMTTFVVCNECGNRWKFC

• Full Name: transcription elongation factor A (SII), 1
• Calculated Molecular Weight: 301 aa, 34 kDa
• Observed Molecular Weight: 40 kDa
• GenBank Accession Number: BC072460
• Gene Symbol: TCEA1
• Gene ID (NCBI): 6917
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23193
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

TCEA1, also named as transcription elongation factor A protein 1, is a 301 amino acid protein, which contains 1 TFIIS central domain and belongs to the TFS-II family. TCEA1 localizes in the nucleus and is necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. The calcualted molecular weight of TCEA1 is 37 kDa, but modified TCEA1 is about 37-40 kDa.