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  • Western Blot (WB) analysis of various lysates using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis using 85704-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis of human heart using 85704-1-RR

    human heart tissue were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using COL6A1 Recombinant antibody (85704-1-RR)

    WB analysis using 85704-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85704-1-RR (COL6A1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of mouse liver using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse liver tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of mouse liver using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 85704-1-RR (Collagen Type VI antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of human colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using COL6A1 Recombinant antibody (85704-1-RR)

    IHC staining of human colon using 85704-1-RR

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 85704-1-RR (COL6A1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

COL6A1 Recombinant antibody

COL6A1 Uni-rAbTM Recombinant Antibody for WB, IHC, ELISA
Cat No. 85704-1-RR
Clone No.242936H3

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

COL6, collagen 6A1, Collagen alpha 1(VI) chain, Collagen alpha-1(VI) chain, Collagen Type VI

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

Positive WB detected inhuman kidney tissue, PC-3 cells, human heart tissue, mouse kidney tissue, mouse heart tissue
Positive IHC detected inmouse liver tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85704-1-RR targets COL6A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen COL6A1 fusion protein Ag11809 Predict reactive species
Full Name collagen, type VI, alpha 1
Calculated Molecular Weight 1028 aa, 109 kDa
Observed Molecular Weight 140 kDa
GenBank Accession NumberBC052575
Gene Symbol COL6A1
Gene ID (NCBI) 1291
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12109
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Type VI Collagen is a major structural component of microfibrils. Type VI Collagen was found to be present throughout the connective tissue and in the extracellular matrix of cultured fibroblasts (PMID: 6723253). It is a heterotrimer composed of three different chains: alpha-1(VI), alpha-2(VI), and alpha-3(VI) or alpha-5(VI) or alpha-6(VI). The alpha-1(VI) chain is encoded by COL6A1 gene. Mutations in this gene result in Bethlem myopathy and Ullrich congenital muscular dystrophy.

Protocols

Product Specific Protocols
WB protocol for COL6A1 antibody 85704-1-RRDownload protocol
IHC protocol for COL6A1 antibody 85704-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols